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Identifying Variables Influencing Traditional Food Solid-State Fermentation by Statistical Modeling
Guangyuan Jin1, Sjoerd Boeschoten2, Jos Hageman3
1The Lab of Brewing Microbiology and Applied Enzymology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Statistical models revealed that lactic acid, not ambient temperature, likely inhibits Chinese liquor yield during solid-state fermentation. Further research is needed to confirm key variables for process improvement.
Area of Science:
- Biotechnology
- Food Science
- Chemical Engineering
Background:
- Traditional solid-state fermentation (SSF) processes often lack scientific basis, leading to unpredictable outcomes in food production.
- Mathematical models struggle to capture the dynamic complexity of SSF, hindering identification of critical factors affecting yield and quality.
Purpose of the Study:
- To develop statistical models correlating industrial data with final Chinese liquor yield in SSF.
- To identify key variables influencing yield fluctuations in traditional Chinese liquor production.
Main Methods:
- Established statistical models using industrial data: initial starch, water, acid content, starting temperature, and substrate temperature profiles.
- Developed a lactic-acid-inhibition model to explain seasonal yield variations.
Main Results:
- Models based solely on initial conditions and temperature profiles provided unsatisfactory yield predictions.
- Seasonal yield differences, particularly the drop from April to December, were not explained by ambient temperature.
- A lactic-acid-inhibition model suggested lactic acid produced by bacteria as a probable cause for reduced yield.
Conclusions:
- Lactic acid accumulation is a likely factor limiting Chinese liquor yield in SSF.
- Integrated microbiological and engineering studies are essential to pinpoint critical variables for SSF optimization.
- Findings offer insights for improving complex solid-state fermentation processes.
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