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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Jun 26, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination.

Ning Xu¹, Sarah E Bohndiek^2,3, Zexing Li⁴

¹State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.

Nature Communications

|May 16, 2024

Summary

This summary is machine-generated.

We developed Mechanical-scan-free multiColor Super-resolution Microscopy (MCoSM) for efficient multicolor super-resolution imaging. This new method offers adjustable resolution and high-quality illumination for nanoscale studies.

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Last Updated: Jun 26, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

Optical microscopy
Nanotechnology
Biophysics

Background:

Point-scanning microscopy is advancing super-resolution imaging.
Existing multifocal techniques enable high-speed imaging but lack efficient multicolor capabilities with high-quality illumination.

Purpose of the Study:

To introduce Mechanical-scan-free multiColor Super-resolution Microscopy (MCoSM).
To enable mechanical-scan-free super-resolution imaging with adjustable resolution and field-of-view.
To provide a versatile platform for nanoscale imaging of molecular interactions.

Main Methods:

Utilized spatial light modulators for diffractive spot array illumination.
Implemented a time-sharing process with different color illumination and phase-shift scanning.
Demonstrated adjustable resolution and field-of-view through varying illumination parameters (100-2,500 s).

Main Results:

Successfully achieved mechanical-scan-free super-resolution imaging.
Showcased adjustable resolution and effective field-of-view.
Performed high-resolution four-color imaging of fluorescent beads.

Conclusions:

MCoSM offers an efficient solution for multicolor super-resolution imaging.
The technique extends spectral imaging capabilities while maintaining spatial flexibility.
MCoSM is a promising platform for nanoscale investigations of complex biological samples.