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Updated: Jun 24, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
Published on: August 15, 2014
Adaptive focus stacking for large depth-of-field microscopic structured-light 3D imaging
Abstract:
This paper presents an adaptive focus stacking method for large depth-of-field (DOF) 3D microscopic structured-light imaging systems. Conventional focus stacking methods typically capture images under a series of pre-defined focus settings without considering the attributes of the measured object. Therefore, it is inefficient since some of the focus settings might be redundant. To address this problem, we first employ the focal sweep technique to reconstruct an initial rough 3D shape of the measured objects. Then, we leverage the initial 3D data to determine effective focus settings that focus the camera on the valid areas of the measured objects. Finally, we reconstruct a high-quality 3D point cloud using fringe images obtained from these effective focus settings by focus stacking. Experimental results demonstrate the success of the proposed method.
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