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Updated: Jun 23, 2025

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Cardiac Development at a Single-Cell Resolution.

Nicholas Wei¹, Carissa Lee¹, Lauren Duan¹

¹Stanford University, Cardiovascular Institute, Stanford, CA, USA.

Advances in Experimental Medicine and Biology

|June 17, 2024

Summary

This summary is machine-generated.

Single-cell RNA sequencing reveals the diverse cell types and gene networks in mammalian heart development. This technology offers new insights into congenital heart diseases and cardiac repair.

Keywords:

Bioinformatics Chamber morphogenesis Heart development Myocardial trabeculation Single-cell RNA sequencing

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Last Updated: Jun 23, 2025

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

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Author Spotlight: Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

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Published on: October 12, 2017

Area of Science:

Cardiovascular Biology
Developmental Biology
Genomics

Background:

Mammalian cardiac development is intricate, with traditional methods offering limited resolution.
Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of cellular heterogeneity.

Purpose of the Study:

To profile the spatiotemporal transcriptomic landscape of the developing mammalian heart.
To identify novel cardiac progenitor populations and understand gene regulatory networks.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) applied to developing human and mouse hearts.
Analysis of transcriptomic data to identify cell diversity and developmental signatures.

Main Results:

Detailed characterization of diverse cardiac cell populations, including myocytes and non-myocytes.
Identification of chamber-specific developmental signatures and gene regulatory networks.
Insights into cardiac conduction system and immune cell populations.

Conclusions:

scRNA-seq provides unprecedented resolution of cardiac development and cell diversity.
Understanding developmental mechanisms aids in studying congenital heart diseases and cardiac regeneration.
Future multiomics approaches promise a more comprehensive cardiac atlas.