Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-isocyanate polyurethane foams: where we stand and what comes next?

Chemical Society reviews·2026
Same author

Harnessing Particle Size Segregation To Tune Molecular Additive Distribution in Coatings.

Industrial & engineering chemistry research·2026
Same author

Synthesis and Processing of Polydepsipeptide- and Polylactic Acid-Based Microparticles with Tunable Degradation Profiles.

Biomacromolecules·2026
Same author

Catalyst Selection for Body-Temperature Curable Polyurethane Networks from Poly(δ-Decalactone) and Lysine Diisocyanate.

Polymers·2025
Same author

Biocompatible Glues: Recent Progress and Emerging Frontiers in Surgical Adhesion.

Polymers·2025
Same author

How to Characterize Covalent Adaptable Networks: A User Guide.

ACS polymers Au·2025

Related Experiment Video

Updated: May 13, 2026

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

7.7K

Understanding associative polymer self-assembly with shrinking gate fluorescence correlation spectroscopy.

Timothy J Murdoch1, Baptiste Quienne2, Julien Pinaud2

  • 1Department of Materials, Loughborough University, LE11 1RJ Loughborough, UK. i.martin-fabiani@lboro.ac.uk.

Nanoscale
|June 18, 2024
PubMed
Summary

This study uses a novel fluorescence method to track polymer self-assembly in liquids. It reveals how hydrophobically modified ethoxylated urethane (HEUR) forms aggregates and networks, crucial for liquid formulations.

More Related Videos

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

11.0K
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

5.6K

Related Experiment Videos

Last Updated: May 13, 2026

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

7.7K
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

11.0K
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

5.6K

Area of Science:

  • Polymer Science
  • Materials Chemistry
  • Physical Chemistry

Background:

  • Polymer self-assembly is critical for liquid formulations.
  • Understanding polymer aggregation dynamics is essential for material design.

Purpose of the Study:

  • To investigate the self-assembly of hydrophobically modified ethoxylated urethane (HEUR) polymers.
  • To apply shrinking gate fluorescence correlation spectroscopy (sgFCS) for polymer dynamics analysis.
  • To characterize the formation of micellar aggregates and percolated networks in HEUR solutions.

Main Methods:

  • Utilized a fluorescence dye sensitive to local nanoviscosity.
  • Employed shrinking gate fluorescence correlation spectroscopy (sgFCS) to isolate bound dye dynamics.
  • Analyzed fluorescence lifetime measurements to correlate with polymer concentration.

Main Results:

  • Identified a small fraction of dye (<1%) strongly bound to HEUR, influencing fluorescence lifetime.
  • sgFCS successfully isolated the diffusional dynamics of the bound dye fraction.
  • Observed micellar aggregate formation between 0.2-1 wt% HEUR, followed by network percolation.

Conclusions:

  • sgFCS offers enhanced sensitivity for studying polymer self-assembly compared to standard FCS.
  • The method is adaptable for any dye exhibiting lifetime changes upon binding.
  • This technique provides new insights into polymer aggregation and network formation in liquid formulations.