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Updated: Jun 23, 2025

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Identification and Characterization of Antigen-Specific T-Cells in Viral Infections

Darya Trubach1, Konjit Getachew Muleta2, Katharina Lahl1,2

  • 1Section for Experimental and Translational Immunology, Institute for Health Technology, Technical University of Denmark (DTU), Kongens Lyngby, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|June 18, 2024
PubMed

Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantification and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.

Keywords:
CD8+ T-cellsCytokine secretion assayFlow cytometryRotavirusSARS-CoV-2T-cell epitopesT-cell stainingTetramers

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