Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...

Clot Retraction and Fibrinolysis

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.

Disorders of Hemostasis

Disorders of Hemostasis

Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

JMJD3 regulates the M2-like macrophage polarization and promotes the growth of breast cancer cells via STAT6/IRF4 axis.

PloS one·2026

Same author

BACH1 impairs hepatocyte regeneration after hepatectomy with repeated ischemia/reperfusion by reprogramming energy metabolism and exacerbating oxidative stress.

Biochemical pharmacology·2024

Same author

Multifunctional chitosan-based hydrogel wound dressing loaded with Acanthopanax senticosus and Osmundastrum cinnamomeum: Preparation, characterization and coagulation mechanism.

Journal of the mechanical behavior of biomedical materials·2024

Same author

Pathogenicity and landscape of differential gene expression in mice orally infected with clinical coxsackievirus A6 (CA6).

Journal of virology·2024

Same author

Intracranial aneurysm rupture risk in northern China: a retrospective case-control study.

Quantitative imaging in medicine and surgery·2024

Same author

Predictive value of serum MDA and 4-HNE levels on the occurrence of early neurological deterioration after intravenous thrombolysis with rt-PA IVT in patients with acute ischemic stroke.

Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association·2024

Same journal

ErNP@SiO2-ICG NIR Probes Enhance Thoracoscopy for Diagnosing Malignant Pleural Effusion in Internal Medicine.

Clinical laboratory·2026

Same journal

Early Coagulation Test Combined with Complete Blood Count in Diagnosis of Pulmonary Contusion and Fracture in Children.

Clinical laboratory·2026

Same journal

Plasma-Derived Exosomal circGAPVD1 Predicts Malignant Bowel Obstruction in Colorectal Cancer.

Clinical laboratory·2026

Same journal

Evaluation of Reference Intervals for Serum Parathyroid Hormone in Korean Adults.

Clinical laboratory·2026

Same journal

Evaluation of Neutrophil-Related Parameters by Hematology Analyzer for Detection of Myelodysplastic Syndromes.

Clinical laboratory·2026

Same journal

Can Pleth Variability Index Levels in Patients with Upper Gastrointestinal Bleeding Predict the Requirement for Transfusion.

Clinical laboratory·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 22, 2025

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

A Case of False Decrease of Plasma D-Dimer.

Minggang Lu, Liangfeng Hu, Lihong Zhang

Clinical Laboratory

|July 5, 2024

Summary

This summary is machine-generated.

Disseminated intravascular coagulation (DIC) can cause a false decrease in D-dimer test results due to the hook effect. Sample dilution can confirm high D-dimer levels and ensure accurate diagnosis.

More Related Videos

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

Published on: June 2, 2015

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

Related Experiment Videos

Last Updated: Jun 22, 2025

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

Published on: June 2, 2015

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood

Published on: October 12, 2012

Area of Science:

Clinical Chemistry
Hematology

Background:

D-dimer is crucial for diagnosing thrombotic diseases and monitoring thrombolysis.
Accurate D-dimer testing is vital for effective patient management.

Observation:

A case of disseminated intravascular coagulation (DIC) presented with a falsely decreased D-dimer level.
Initial D-dimer results were 1.09 mg/L, 0.93 mg/L, and 1.43 mg/L.

Findings:

High D-dimer concentrations exceeding the assay's detection range caused a hook effect.
Dilution of samples revealed significantly higher D-dimer levels (e.g., 842.24 mg/L, 1,505.28 mg/L) consistent with DIC.
The hook effect led to a false decrease in D-dimer values before dilution.

Implications:

Clinicians should consider the hook effect when D-dimer results are incongruent with the clinical presentation in DIC patients.
Sample dilution is a reliable method to rule out false decreases and obtain accurate D-dimer measurements.
Accurate D-dimer testing is essential to prevent diagnostic delays and ensure timely treatment for DIC.