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Updated: Jun 18, 2025
Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
Published on: April 1, 2014
Bacterial Opsonization Changes Adhesion Interactions between Endothelial Cells and Neutrophils in a Model of
S N Pleskova1,2, S Z Bobyk3, N A Bezrukov3
1Research Laboratory of Scanning Probe Microscopy, Scientific Education Center Physics of Solid-State Nanostructures, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia. pleskova@mail.ru.
Abstract:
The influence of non-opsonized and opsonized S. aureus 2879M and E. coli 321 strains on the total strength of interaction between the endothelial cell and neutrophil during the docking process was studied using in vitro model of experimental septicemia. We observed a decrease in the force and work of adhesion between receptors of neutrophils and endothelial cells under the influence of non-opsonized strains and further decrease in the affinity of single interactions between cells under the influence of opsonized S. aureus, which was compensated by an increase in the number of contacts, as well as an increase in the force of adhesion under the influence of opsonized E. coli compared to non-opsonized bacteria, which remained below the control level, while adhesion work reaches the control level. Thus, opsonization of S. aureus aggravates the "immunological uncoupling" between neutrophils and endothelial cells, while opsonization of E. coli reduces the pathological effect compared to non-opsonized bacteria.
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