PROTECTIVE EFFECT OF A NEW SUPEROXIDE-PRODUCING ENZYME COMPLEX FROM RASPBERRY IN RATS WITH THIRD-DEGREE THERMAL BURNS

Area of Science:

• Biochemistry and Molecular Biology
• Dermatology and Wound Healing
• Regenerative Medicine

Background:

• Thermal burns, particularly third-degree, necessitate advanced treatments for effective wound healing.
• Accelerating burn wound regeneration is a critical challenge in modern medical research.
• Oxygen radicals are vital for homeostasis, immune response, and tissue regeneration.

Purpose of the Study:

• To evaluate the reparative effects of a superoxide (O2-)-producing enzyme (SPE) from raspberries on third-degree thermal burn wounds.
• To assess the impact of SPE application on hematological, cytogenetic, and histological parameters in burn-injured animal models.

Main Methods:

• Third-degree thermal burns were induced in animal models.
• A superoxide (O2-)-producing enzyme (SPE) from raspberries was applied topically and injected at 12.75% and 5% concentrations.
• Hematological parameters, bone marrow cytogenetic indices (mitotic index, polyploid cells, chromosomal aberrations), and wound area were analyzed.

Main Results:

• SPE application, particularly the 12.75% solution, significantly reduced burn wound area compared to controls.
• The 12.75% SPE solution resulted in 100% survival rate, while the 5% solution yielded 50% survival over 30 days.
• SPE treatment led to normalized hematological parameters and a tendency toward normalization of the mitotic index, with reduced polyploid cells and chromosomal aberrations.

Conclusions:

• A 12.75% superoxide (O2-)-producing enzyme (SPE) solution derived from raspberries demonstrates significant efficacy in promoting the healing of third-degree thermal burns.
• SPE application positively influences hematological and cytogenetic parameters, suggesting a role in tissue regeneration and reduced cellular damage.
• Raspberry-derived SPE shows promise as a novel therapeutic agent for accelerating burn wound healing and improving patient outcomes.