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Electrochemical Detection of Nucleic Acids Using Three-Dimensional Graphene Screen-Printed Electrodes
Dar Tafazul Islam1, Shariat Mobasser1, Sruthi Kotaru2,3
1Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 5, 2024
Summary
Screen-printed electrodes (SPEs) offer sensitive detection of nucleic acid biomarkers. This electrochemical approach enables quantification of DNA and RNA at attomolar levels without amplification.
Area of Science:
- Electrochemistry
- Biosensors
- Nucleic Acid Detection
Background:
- Electrochemical methods and electrode miniaturization are vital for detecting nucleic acid biomarkers.
- Screen-printed electrodes (SPEs) and printed circuit boards facilitate electrode miniaturization.
- Various electrode materials like glassy carbon, platinum, carbon, and graphene are utilized in SPEs.
Purpose of the Study:
- To describe the fundamental approach for utilizing SPEs in nucleic acid measurements.
- To highlight the advantages of electrochemical detection for biomarker quantification.
Main Methods:
- Utilizing miniaturized electrodes, specifically screen-printed electrodes (SPEs).
- Employing potentiostats for electrochemical measurements.
- Exploring various electrode materials and surface modifications.
Main Results:
- Demonstrated superior limit of detection for biomarkers without the need for amplification.
- Achieved detection of DNA and RNA concentrations in the nanomolar (nM) to attomolar (aM) range.
- Successful application of SPEs for detecting cells, proteins, DNA, and RNA.
Conclusions:
- SPEs provide a powerful and sensitive platform for electrochemical nucleic acid detection.
- This technique offers high sensitivity and broad applicability for biomarker quantification.
- The described approach is fundamental for advancing nucleic acid measurement technologies.
