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Protocol for isolating high-purity natural killer cells from peripheral blood of septic patients using magnetic cell
Anna Coulibaly1, Timo Sturm1, Jutta Schulte1
1Department of Anesthesiology, Surgical Intensive Care Medicine and Pain Medicine, Mannheim Institute of Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
Abstract:
In human sepsis, myelocytosis and concomitant lymphopenia complicate the study of peripheral blood natural killer (NK) cells. Here, we present a protocol for isolating NK cells from peripheral blood of septic patients using magnetic cell separation. We describe steps for the depletion of non-NK cells and NK cell enrichment. We then detail procedures for comparing the results from this protocol to results obtained through the isolation procedures using two commercially available kits for NK cell isolation. For complete details on the use and execution of this protocol, please refer to Coulibaly et al.1.
