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Updated: Jun 13, 2025

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Musketeer: a software tool for the analysis of titration data
Daniil O Soloviev1, Christopher A Hunter1
1Yusuf Hamied Department of Chemistry, University of Cambridge Lensfield Road Cambridge CB2 1EW UK herchelsmith.orgchem@ch.cam.ac.uk.
Abstract:
Musketeer is a powerful open-source software tool for the analysis of titration data, featuring a simple cross-platform graphical interface for importing data directly from UV-vis, fluorescence and NMR spectrometers, or from spreadsheets. The fast data analysis algorithm can be used to obtain equilibrium constants for simple binding isotherms, as well as for more complicated systems with multiple competing equilibria. Applications of Musketeer for the analysis of a range of different supramolecular and biomolecular systems are illustrated, including titrations with multiple spectroscopically active species, competitive binding assays, denaturation experiments, optimisation of concentrations as variables. The software also includes a number of tools that can be used to select the binding isotherm that represents the best model to describe a dataset.
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