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Quantifying Phospholipids in Organic Samples Using a Hydrophilic Interaction Liquid Chromatography-Inductively
Wladimir Ruiz1,2,3, Stéphanie Fontagné-Dicharry4, Sylvain Verdier5
1Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM, UMR 5254, 2 Av. Pr. Angot, 64053 Pau, France.
Analytical Chemistry
|September 12, 2024
Summary
A new method quantifies phospholipids in oils using hydrophilic interaction liquid chromatography (HILIC) and inductively coupled plasma high-resolution mass spectrometry (ICP-HRMS). This streamlined approach eliminates extensive sample preparation, improving lipid analysis accuracy.
Area of Science:
- Analytical Chemistry
- Lipidomics
Background:
- Phospholipids are crucial lipid components in various biological and industrial samples.
- Accurate quantification of phospholipids in complex matrices like oils remains challenging.
- Existing methods often require extensive sample preparation, increasing time and cost.
Purpose of the Study:
- To develop and validate a novel, streamlined method for direct phospholipid quantification in oil samples.
- To utilize hydrophilic interaction liquid chromatography (HILIC) coupled with inductively coupled plasma high-resolution mass spectrometry (ICP-HRMS).
- To assess the method's applicability across diverse oil matrices.
Main Methods:
- Employed a bridged ethyl hybrid (BEH) HILIC column with a tetrahydrofuran/water mobile phase.
- Utilized inductively coupled plasma high-resolution mass spectrometry (ICP-HRMS) for detection and quantification.
- Experimentally compensated for gradient/matrix effects on ICP-HRMS sensitivity.
Main Results:
- Successfully quantified phospholipids directly in various oil samples, including vegetable oils, animal fats, and supplements.
- Demonstrated the elimination of the need for prior sample preparation steps like solid phase extraction (SPE).
- Achieved high precision and accuracy in phospholipid quantification.
Conclusions:
- The developed HILIC-ICP-HRMS method offers a robust and efficient approach for direct phospholipid analysis in oils.
- This advancement simplifies lipid analysis, reducing sample preparation burdens.
- The method has significant implications for quality control and research in food, nutrition, and renewable energy sectors.
