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Labeling DNA Probes

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Updated: Jun 12, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

A versatile bioluminescent probe with tunable color.

Zachary R Torrey¹, Lila P Halbers², Lorenzo Scipioni³

¹Department of Chemistry, University of California Irvine Irvine CA 92697 USA jpresche@uci.edu.

RSC Chemical Biology

|September 23, 2024

Summary

This summary is machine-generated.

Researchers developed a new bioluminescence imaging platform, BREAKFAST, for microscale visualization. This tool enables dynamic, multi-color imaging at the cellular level, advancing in vivo studies.

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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

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Last Updated: Jun 12, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

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Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

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A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence FUEL

Published on: May 23, 2014

Area of Science:

Biotechnology
Molecular Imaging
Cell Biology

Background:

Bioluminescence imaging is valuable for in vivo studies but limited at the microscale due to a lack of versatile tools for dynamic event visualization.
Current methods often lack the flexibility required for detailed cellular-level imaging.

Purpose of the Study:

To develop a novel platform for microscale bioluminescence imaging.
To create a versatile tool for visualizing dynamic biological events with enhanced spectral capabilities.

Main Methods:

Development of the Bioluminescence Resonance Energy mAKe over with a Fluorescence-Activating absorption-Shifting Tag (BREAKFAST) platform.
Integration of a bright luciferase with a chemogenetic tag (pFAST) for rapid color switching.
Utilizing resonance energy transfer with luciferin and fluorogenic ligands for signal detection and spectral analysis.

Main Results:

Established the utility of BREAKFAST for combined fluorescence and bioluminescence imaging.
Achieved dynamic, four-color visualization through sequential ligand addition and spectral phasor analysis.
Demonstrated selective signal quenching using a dark fluorogen, showcasing precise control over imaging signals.

Conclusions:

BREAKFAST provides a new method for cellular-scale bioluminescence imaging.
This platform enhances the visualization of dynamic events at the microscale.
The developed technology lays the groundwork for future advancements in bioluminescence probe development and imaging applications.