Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
RNA Splicing01:32

RNA Splicing

56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inosine incorporation in DNA nanostructures and 3D DNA crystals.

bioRxiv : the preprint server for biology·2026
Same author

Aptamer-based DNA nanoswitches for multiplexed protein detection.

bioRxiv : the preprint server for biology·2026
Same author

Paranemic Cohesion of DNA under Isothermal Conditions.

JACS Au·2026
Same author

Light-responsive DNA nanostructures.

Nature reviews. Chemistry·2026
Same author

DNA Nanostructures for siRNA Delivery.

Bioconjugate chemistry·2026
Same author

Left-Handed Helices in DNA Nanotechnology.

JACS Au·2026
Same journal

Lipid Metabolic Labeling to Study Site- and Lipid-Specific Long-Chain <i>S</i>-Acylation Dynamics.

ACS chemical biology·2026
Same journal

Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

ACS chemical biology·2026
Same journal

Synthesis and Characterization of the Spectroscopic and Imaging Utilities of Two Indole-Based Cyan Fluorescent Nucleoside Analogues.

ACS chemical biology·2026
Same journal

Indole Ring Expansion and Rearrangement-Enabled Quinoline Scaffold Formation in the Biosynthesis of the Antitumor Monoterpene Indole Alkaloid Camptothecin.

ACS chemical biology·2026
Same journal

Intracellular Delivery of Peptides and Proteins with an Engineered Membrane Translocation Domain.

ACS chemical biology·2026
Same journal

Development of Next-Generation Fluoroacetamidine-Containing Activity-Based Probes for the Selective Labeling of the Protein Arginine Deiminases (PADs).

ACS chemical biology·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K

Switchback RNA.

Bharath Raj Madhanagopal1, Hannah Talbot1, Arlin Rodriguez1

  • 1The RNA Institute, University at Albany, State University of New York, Albany, New York 12222, United States.

ACS Chemical Biology
|September 24, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed switchback RNA, a novel motif for nucleic acid nanostructures. This motif demonstrates enhanced stability and unique properties for applications in RNA nanotechnology and small molecule loading.

More Related Videos

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.0K

Related Experiment Videos

Last Updated: Jun 12, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using &#967;CRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Nucleic acid nanostructures rely on specific DNA and RNA motifs for assembly and function.
  • Developing novel RNA motifs is crucial for advancing RNA nanotechnology.

Purpose of the Study:

  • To introduce and characterize a novel, globally left-handed RNA motif termed 'switchback RNA'.
  • To explore its assembly, biophysical properties, and potential applications in RNA nanotechnology.

Main Methods:

  • Assembly of switchback RNA in various buffer conditions (with and without Mg2+, Na+, K+).
  • Biophysical characterization including thermal stability assessments.
  • Biochemical assays to evaluate small molecule binding and nuclease resistance.
  • Strand displacement assays to assess differential strand affinity.

Main Results:

  • Switchback RNA can be assembled without Mg2+ and shows enhanced thermal stability with Mg2+, Na+, or K+.
  • Distinct small molecule binding compared to conventional RNA suggests potential for targeted loading.
  • Enabled toehold-less strand displacement due to differential strand affinities.
  • Exhibited comparable nuclease resistance to conventional RNA structures.

Conclusions:

  • Switchback RNA is a versatile motif for constructing stable RNA nanostructures.
  • Its unique properties offer new design strategies for small molecule delivery and toehold-less strand displacement.
  • The stability in low magnesium conditions warrants further investigation into its natural occurrence.