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Updated: Jun 12, 2025
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
A novel aptasensor based endogenous enzyme-powered DNA walker for ATP imaging in specific living cells
Chunrong Li1, Tong Li2, Mingqi Guo2
1Qiannan Medical College for Nationalities, Duyun, 558000, China. 327854055@qq.com.
Abstract:
Highly sensitive and specific imaging of ATP in living cells remains a challenge. Here, a novel aptasensor based endogenous enzyme-powered DNA walker for imaging ATP was proposed. The strategy leverages the highly expressed APE1 in tumor cells as the driving force of the DNA walker, achieving high sensitivity and superior imaging contrast. The method can detect ATP as low as 3.43 μM within 1 h. The approach can also effectively monitor intracellular ATP expression fluctuations and successfully differentiate between normal and cancer cells with high contrast.
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