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Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

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Bioactivated Glucoraphanin Modulates Genes Involved in Necroptosis on Motor-Neuron-like Nsc-34: A Transcriptomic

Aurelio Minuti¹, Alessandra Trainito¹, Agnese Gugliandolo¹

¹IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy.

Antioxidants (Basel, Switzerland)

|September 28, 2024

Summary

Sulforaphane (RS-GRA) shows potential for treating neurodegenerative diseases by boosting redox activity and inhibiting necroptosis in nerve cells. This research explored its effects on gene expression, suggesting optimal dosing strategies.

Keywords:

glucosinolates isothiocyanates necroptosis oxidative stress pathway analysis transcriptomic analysis

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Area of Science:

Neuroscience
Molecular Biology
Pharmacology

Background:

Bioactive compounds are increasingly studied for health benefits, especially in chronic and neurodegenerative diseases.
Sulforaphane (RS-GRA) is a compound of interest for its potential therapeutic properties.
Understanding its molecular mechanisms is crucial for developing new treatments.

Purpose of the Study:

To investigate the in vitro effects of sulforaphane (RS-GRA) on differentiated NSC-34 cells.
To identify molecular pathways modulated by RS-GRA exposure.
To determine the impact of different concentrations and durations of RS-GRA treatment.

Main Methods:

Transcriptomic analysis was performed on NSC-34 cells treated with RS-GRA.
Cells were exposed to varying concentrations (1 µM, 5 µM, 10 µM) and time points (24h, 48h, 72h).
Differential gene expression and pathway perturbation analyses were conducted.

Main Results:

39 genes showed consistent differential expression across all tested conditions.
Key genes involved in redox balance (e.g., Nfe2l2, Sod1, Sod2) were upregulated.
Genes associated with necroptosis (e.g., Ripk1, Ripk3, Mlkl) were downregulated, with significant pathway effects observed.

Conclusions:

RS-GRA significantly enhances redox pathway activity and inhibits necroptosis in neuronal cells.
These findings support RS-GRA's potential as an adjuvant therapy for neurodegenerative conditions.
Data suggest both acute (high dose) and chronic (low dose) administration strategies may be effective.