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[Interactions between Neisseria meningitidis and cell cultures (data of cytomorphologic studies)]
Abstract:
The present work shows that the cytopathogenic action of N. meningitidis on continuous human amniotic epithelial cell culture FL begins from their active adhesion and subsequent invasion. The degree of the manifestation of the cytopathic effect depends on the capacity of the infective agent for adhesion and invasion and on its biological properties, as well as on the initial state of the cells. The infection of the cells is accompanied by disturbances in their mitotic activity together with the lesions of their chromosomal apparatus. The cells die either in the state of degenerative mitosis, or as the result of the rupture of the cytoplasm in massively invaded cells. The response of the cells to the invasion of faintly cytopathogenic and noncytopathogenic strains takes the form of nonprofessional phagocytosis.
Insights
Neisseria meningitidis (N. meningitidis) invades human amniotic cells, causing cell damage and death. Cell death mechanisms include errors in cell division and rupture, with varying effects based on bacterial strain and cell condition.
Area of Science:
- Microbiology
- Cell Biology
- Pathogenesis
Background:
- Neisseria meningitidis (N. meningitidis) is a significant human pathogen.
- Understanding the initial interactions between N. meningitidis and host cells is crucial for elucidating disease mechanisms.
Purpose of the Study:
- To investigate the cytopathogenic effects of N. meningitidis on human amniotic epithelial cells (FL cell line).
- To characterize the cellular responses to N. meningitidis invasion, including effects on cell division and structure.
Main Methods:
- Utilized a continuous human amniotic epithelial cell culture (FL cell line).
- Observed the cytopathic effects of N. meningitidis infection, focusing on adhesion, invasion, and cellular damage.
- Analyzed mitotic activity and chromosomal integrity in infected cells.
Main Results:
- N. meningitidis initiates cytopathic effects through active adhesion and invasion of FL cells.
- The extent of cell damage correlates with the bacteria's invasive capacity and biological properties, as well as the host cell's state.
- Infection led to disturbances in mitotic activity and chromosomal aberrations.
- Cell death occurred via degenerative mitosis or cytoplasmic rupture in heavily infected cells.
- Less cytopathogenic strains elicited nonprofessional phagocytosis.
Conclusions:
- N. meningitidis actively invades and damages human amniotic epithelial cells, leading to characteristic cytopathic effects and cell death.
- Host cell responses vary depending on the N. meningitidis strain's virulence and the host cell's condition.