Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

732
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
732
Complement System01:27

Complement System

2.3K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
2.3K
Nervous Tissue: Myelin01:25

Nervous Tissue: Myelin

2.4K
The myelin sheath is a multilayered lipid and protein covering that insulates the axon of a neuron, enhancing the speed of nerve impulse conduction. Axons without this sheath are referred to as unmyelinated. Two types of neuroglia, Schwann cells in the peripheral nervous system (PNS) and oligodendrocytes in the central nervous system (CNS) are responsible for producing myelin sheaths.
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...
2.4K
Neurons: The Axon01:21

Neurons: The Axon

3.3K
Axons are long, cytoplasmic processes of nerve cells capable of propagating electrical impulses known as action potentials. The cytoplasm or axoplasm of an axon contains neurofibrils, neurotubules, small vesicles, lysosomes, mitochondria, and various enzymes, all encased within the axolemma, the plasma membrane of the axon.
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment....
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Do Collagenated Xenogenic Bone Substitutes Enhance Gingival Healing and Angiogenesis Through a Barrier Membrane? An In Vitro Study.

Materials (Basel, Switzerland)·2026
Same author

Microbubbles for Acoustically Mediated Drug Delivery to the Inner Ear.

Molecular pharmaceutics·2026
Same author

Macrophage-Pulp Fibroblast Interactions Modulate Initial Dental Pulp Regeneration In Vitro.

International endodontic journal·2026
Same author

Neuroinflammatory Consequences of Rhinovirus Infection in Human Epithelial and Neuronal Models.

Lung·2025
Same author

Tissue-Engineered Oral Epithelium for Dental Material Testing: Toward <i>In Vitro</i> Biomimetic Models.

Tissue engineering. Part C, Methods·2024
Same author

Immunological isolation and characterization of neuronal progenitors from human dental pulp: A laboratory-based investigation.

International endodontic journal·2024
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
Same journal

PACAP and Maxadilan (PAC1 Agonist) Influence Plaque Progression, Migratory Ability, and Mitochondrial Morphology and Dynamics in Vascular Smooth Muscle Cells.

Cells·2026
See all related articles

Related Experiment Video

Updated: Jun 9, 2025

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
11:18

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Published on: November 25, 2014

10.8K

Complement C5a Implication in Axonal Growth After Injury.

Aurélie Cotten1, Charlotte Jeanneau1, Patrick Decherchi1

  • 1Aix-Marseille University, CNRS, ISM, 13009 Marseille, France.

Cells
|October 25, 2024
PubMed
Summary
This summary is machine-generated.

Complement C5a protein enhances axonal regeneration after injury. This discovery offers a potential new therapeutic strategy for treating central nervous system damage and restoring lost functions.

Keywords:
Complement C5aaxon regenerationaxotomyneuron injury

More Related Videos

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
08:52

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Published on: March 30, 2018

8.7K
Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

13.0K

Related Experiment Videos

Last Updated: Jun 9, 2025

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
11:18

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Published on: November 25, 2014

10.8K
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
08:52

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Published on: March 30, 2018

8.7K
Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
10:45

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

13.0K

Area of Science:

  • Neuroscience
  • Immunology
  • Regenerative Medicine

Background:

  • Complement C5a protein and its receptor (C5aR) are crucial for tissue regeneration.
  • The nervous system expresses C5aR, but effective treatments for nerve injury are lacking.
  • Investigating C5a's role in neural repair is essential.

Purpose of the Study:

  • To explore the effect of Complement C5a on axonal growth following axotomy in vitro.
  • To determine if C5a influences neuronal repair mechanisms.

Main Methods:

  • Primary hippocampal neurons from Wistar rats were cultured.
  • C5aR mRNA expression was confirmed using RT-PCR.
  • Immunofluorescence was used to analyze C5aR membrane expression, localization, and phosphorylation.
  • A 3D-printed microfluidic device was employed to study axonal regeneration after injury.
  • C5a's impact on axonal growth speed and length was quantified.

Main Results:

  • Mature neurons (93%) and astrocytes (7%) comprised the primary cultures.
  • C5aR was found to co-localize with neurofilament light (NF-L) in mature neurons.
  • C5a induced C5aR phosphorylation in these neurons.
  • Application of C5a significantly accelerated axonal growth speed and increased axonal length post-axotomy.

Conclusions:

  • Complement C5a plays a novel role in promoting axonal regeneration after injury.
  • C5a enhances axonal growth, suggesting a potential therapeutic application for central nervous system injuries.
  • This finding opens new avenues for developing treatments to restore function after neural damage.