Related Experiment Video
Updated: Jun 8, 2025
06:15
Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
11.9K
Deep eutectic supramolecular polymers based HPLC stationary phase: Green synthesis strategy and promising application
Qiaoling Liu1, Wanjiao Wei1, Yanjuan Liu2
1School of Chemistry and Environmental Engineering, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430205, China.
Analytica Chimica Acta
|November 3, 2024
Summary
Researchers developed a novel deep eutectic supramolecular polymer (DESP) HPLC stationary phase. This green synthesis method offers effective separation of diverse analytes, including steroid hormones in real samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Polymer Chemistry
Background:
- Deep eutectic solvents (DESs) are cost-effective, biodegradable materials with diverse applications.
- Deep eutectic supramolecular polymers (DESPs) combine DES properties with supramolecular polymer characteristics, showing potential in separation science.
- DESPs have not been previously explored as stationary phases for High-Performance Liquid Chromatography (HPLC).
Purpose of the Study:
- To develop and characterize a novel DESP-based stationary phase for HPLC.
- To evaluate the separation capabilities of the new stationary phase for various analytes.
- To demonstrate the application of the DESP stationary phase in the analysis of steroid hormones in real samples.
Main Methods:
- Green synthesis of a DESP stationary phase (Poly(DES)@SiO2) using DES as both monomer and reaction medium.
- Evaluation of the Poly(DES)@SiO2 column's interaction mechanisms and separation selectivity for hydrophilic and hydrophobic substances.
- Assessment of column efficiency, mechanical stability, and separation repeatability compared to commercial C18 columns.
Main Results:
- The Poly(DES)@SiO2 column demonstrated selective separation of structurally similar hydrophilic/hydrophobic substances via multiple interaction mechanisms.
- Separation of hydrophobic analytes was faster and required less organic eluent compared to a C18 column, albeit with slightly lower efficiency.
- The column exhibited excellent mechanical stability and good repeatability for separating steroid hormones, with recoveries between 94.56% and 103.84% in actual samples.
Conclusions:
- This study presents a green and facile strategy for synthesizing novel DESP-based stationary phases.
- The feasibility of using DESPs for effective HPLC separations has been verified.
- DESP-based stationary phases show promising potential for analyzing complex samples, expanding DES applications in separation science.
![Solid-phase Synthesis of [4.4] Spirocyclic Oximes](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F58508.jpg&w=3840&q=50)
Related Concept Videos
01:11High-Performance Liquid Chromatography: Introduction
1.5K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
1.5K
01:18Supercritical Fluid Chromatography
210
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
SFC utilizes a supercritical fluid mobile phase,...
210
01:05High-Performance Liquid Chromatography: Elution Process
429
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
429