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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

296
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
296
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

313
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
313

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Updated: Jun 8, 2025

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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Harnessing Raman spectroscopy for cell therapy bioprocessing.

Marta H G Costa1, Inês Carrondo1, Inês A Isidro1

  • 1iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.

Biotechnology Advances
|November 3, 2024
PubMed
Summary
This summary is machine-generated.

Raman spectroscopy offers real-time, non-destructive monitoring for cell therapy manufacturing. This process analytical technology (PAT) tool enhances efficiency, ensures product quality, and supports adaptive process control in bioprocessing.

Keywords:
Bioprocess controlBioreactorsCell therapy manufacturingCritical process parametersCritical quality attributesProcess analytical technology

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Area of Science:

  • Biotechnology and Bioprocessing
  • Analytical Chemistry
  • Cell Therapy Manufacturing

Background:

  • Cell therapy production demands precise monitoring of critical parameters for quality and cost-effectiveness.
  • Conventional analytical methods lack real-time insights, hindering process optimization.
  • Process Analytical Technology (PAT) integration is crucial for advancing cell therapy manufacturing.

Purpose of the Study:

  • To review the role of Raman spectroscopy in cell therapy bioprocessing.
  • To highlight its potential for real-time, on-line monitoring and adaptive process control.
  • To discuss its application across various manufacturing stages and downstream processes.

Main Methods:

  • Integration of Raman spectroscopy as a Process Analytical Technology (PAT) tool.
  • On-line, non-destructive measurement of critical parameters and quality attributes.
  • Simultaneous monitoring of cell density, viability, metabolites, and biomarkers.

Main Results:

  • Raman spectroscopy enables continuous data acquisition for real-time correlations.
  • Facilitates streamlined on-line monitoring and adaptive process control.
  • Demonstrates utility from upstream optimization to downstream quality control, including contamination detection.

Conclusions:

  • Raman spectroscopy significantly enhances efficiency and reliability in cell therapy manufacturing.
  • It supports stringent regulatory requirements and mitigates process-related risks.
  • This technology has the potential to revolutionize advanced therapy medicinal product development.