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Modified Hank's Balanced Salt Solution as a Storage Medium for Avulsed Teeth: In Vitro Assessment of Periodontal
Zhi Jin Lee1, Sook Luan Ng2, Eason Soo1
1Unit of Endodontology and Endodontics, Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia (UKM), Kuala Lumpur, Malaysia.
Summary
Adding Vitamin C to Hank's Balanced Salt Solution (HBSS) significantly improved periodontal ligament fibroblast (PDLF) viability for avulsed teeth. HBSS with Vitamin C offers a practical and effective storage medium for tooth replantation.
Area of Science:
- Dental Traumatology
- Biomaterials Science
- Cell Biology
Background:
- Optimal storage of avulsed teeth is crucial for periodontal ligament fibroblast (PDLF) viability.
- Preserving PDLF enhances periodontal fiber reattachment and tooth replantation prognosis.
- Current storage media require evaluation for their efficacy in maintaining cell viability.
Purpose of the Study:
- To compare the efficacy of various storage media in preserving PDLF viability.
- To evaluate Hank's Balanced Salt Solution (HBSS) and its modifications against Dulbecco's Modified Eagle Medium (DMEM).
- To identify an optimal, practical storage medium for avulsed teeth.
Main Methods:
- Human periodontal tissues were used to culture PDLF.
- PDLF cells were exposed to six media: HBSS, HBSS + Vitamin C, HBSS + PDGF, HBSS + PDGF + Vitamin C, HBSS + Platelet Lysate, and DMEM.
- Cell viability was assessed using MTT assay at 3, 6, 12, and 24 hours.
Main Results:
- Vitamin C-supplemented media (HBSS + Vit C, HBSS + PDGF + Vit C) maintained significantly higher PDLF viability compared to HBSS + PDGF and HBSS + PL.
- All tested media, except potentially plain HBSS, showed higher PDLF viability over time compared to baseline.
- DMEM demonstrated the highest cell proliferation but is impractical for clinical use.
Conclusions:
- Supplementation with Vitamin C significantly enhances PDLF viability in HBSS.
- HBSS + Vitamin C presents a viable and practical alternative to DMEM for avulsed tooth storage.
- Optimized HBSS formulations can improve outcomes for tooth replantation procedures.
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