Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Incidence of colonization and infection with carbapenemase-producing Enterobacterales in Toronto and Peel Region, Ontario, 2007-2024.

Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada·2026

Same author

Factors associated with severe health outcomes among community-dwelling older adults hospitalized with respiratory syncytial virus.

The Journal of infection·2026

Same author

A decoy virus strategy using plant-produced SARS-CoV-2 virus-like-particles to inhibit infection and lung injury.

Journal of translational medicine·2026

Same author

Contrast-enhanced MRI improves the diagnostic performance for vaginal fornix invasion in cervical carcinoma.

Insights into imaging·2026

Same author

AMMI Canada seasonal influenza guideline update 2025-26: Some questions answered.

Journal of the Association of Medical Microbiology and Infectious Disease Canada = Journal officiel de l'Association pour la microbiologie medicale et l'infectiologie Canada·2026

Same author

Patient representation in RNA signature studies for detecting and predicting the outcomes of febrile illnesses: a scoping review.

BMC infectious diseases·2026

Same journal

Silicon-Mediated Laser Shock Synthesis of Nanocrystalline Diamonds from Low-Rank Coal.

ACS nano·2026

Same journal

Precursor-Engineered Strategy for Constructing Supported Tetra-Atom Pt Clusters to Boost Propane Dehydrogenation under Direct Resistive Heating.

ACS nano·2026

Same journal

Enterohepatic Circulation of Polystyrene Nanoplastics Promotes Intestinal Inflammation by Impairing Enteric Neurons.

ACS nano·2026

Same journal

Triboelectric Spectroscopy for Identification of Metal Ion Valence States in Aqueous Solutions.

ACS nano·2026

Same journal

Beyond the Continuum Theory: Conductance Scaling and Correlated Imaging in Atom-Scale Artificial Ion Channels.

ACS nano·2026

Same journal

Selenium-Induced Directional Growth of Ultrathin Nanowires with Subnano Amorphous Shells for High-Performance Multifunctional Electrocatalysis.

ACS nano·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 21, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

QBox: An Automated Portable System for Multiplex Quantum Dot Barcode Diagnostics.

Matthew Osborne^1,2, Hongmin Chen^1,2, Pranav Kadhiresan^1,2

¹Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3E2, Canada.

|November 25, 2024

Summary

This summary is machine-generated.

A new portable system automates quantum dot barcode assays for rapid, multiplexed pathogen detection. This technology enhances point-of-care diagnostics, offering potential for infectious diseases, cancer, and genetic conditions.

Keywords:

assay automation barcoding diagnostics multiplex point-of-care quantum dots

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Related Experiment Videos

Last Updated: May 21, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Area of Science:

Biotechnology
Molecular Diagnostics
Point-of-Care Testing

Background:

The COVID-19 pandemic spurred automated molecular detection for point-of-care use.
Current systems face multiplexing limitations due to hardware constraints.
Quantum dot barcodes offer multiplexing but require complex, multi-step assays.

Purpose of the Study:

To develop a portable, automated system for quantum dot barcode assays.
To overcome limitations of existing multiplexed diagnostic technologies.
To enable rapid, on-site detection of multiple molecular targets.

Main Methods:

Engineered a portable cartridge-and-instrument system.
Automated nucleic acid extraction, reverse transcription, amplification, and detection.
Utilized quantum dot barcodes for multiplexed signal generation.

Main Results:

Achieved a complete workflow in 40 minutes.
Clinically validated with SARS-CoV-2 samples (92% sensitivity, 100% specificity).
Demonstrated multiplexing with respiratory (4-barcode) and bloodborne pathogen (5-barcode) panels.

Conclusions:

The portable system facilitates broad application of quantum dot barcodes.
Enables rapid, multiplexed detection of infectious pathogens.
Shows potential for diagnosing cancer and other genetic diseases.