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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 5, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Single-Cell Isolation Chip Integrated with Multicolor Barcode Array for High-Throughput Single-Cell Exosome Profiling

Chao Wang¹, Yu Zhang^1,2, Jianbo Wang³

¹Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.

Advanced Materials (Deerfield Beach, Fla.)

|December 11, 2024

Summary

This summary is machine-generated.

A new platform enables single-cell analysis of exosomes, revealing crucial differences in cancer cell subgroups. This technology helps understand exosome heterogeneity and its role in tumor progression and metastasis.

Keywords:

exosome profiling microfluidic chip photothermal‐driven isolation single cell

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

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Last Updated: Jun 5, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Published on: May 23, 2018

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Area of Science:

Biochemistry
Molecular Biology
Cancer Research

Background:

Exosomes are key biomarkers in cancer progression, influencing tumor formation, growth, and metastasis.
Current bulk exosome detection methods lose parent cell information and ignore exosome heterogeneity.
Analyzing single-cell exosome functional heterogeneity is crucial for understanding cancer.

Purpose of the Study:

To develop a high-throughput platform for single-cell isolation and multi-color exosome phenotype analysis.
To quantify trace exosomes secreted by single cells.
To reveal functional heterogeneity of exosomes from diverse cancer cell subgroups.

Main Methods:

Photothermal-driven single-cell chips for efficient single-cell isolation (≈97% efficiency in 5 min).
Mass spectrometry and protein interaction analysis of breast cancer exosome phenotypic proteins.
Ultra-high throughput analysis of tens of thousands of single cells from breast cancer cell lines and clinical tissues.

Main Results:

Identification of key exosome phenotypes, including CD44 and EGFR co-expressing subgroups in breast cancer cell lines.
Discovery of immune-evasion PD-L1 high-phenotype exosome subgroups in complex tumor microenvironments, particularly in HER2-positive tissues.
Demonstration of significant subgroup differences in exosome expression profiles.

Conclusions:

The developed platform enables powerful single-cell isolation, exosome quantification, and phenotypic analysis.
This technology advances the understanding of single-cell exosome heterogeneity in cancer research.
The findings provide insights into exosome roles in tumor progression and immune evasion.