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Advancing Transfusion Medicine through Raman Tweezers Spectroscopy: A Review of Recent Progress and Future
Mithun Nelliat1, Ganesh Mohan2, Jijo Lukose1
1Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, India.
Summary
Raman tweezers spectroscopy (RTS) offers a powerful method to analyze blood cell quality in transfusion medicine. This technique monitors cellular changes, providing insights into storage lesions and the effects of various treatments on blood products.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Hematology
Background:
- Raman tweezers spectroscopy (RTS) integrates optical tweezers and Raman spectroscopy for single living cell analysis.
- Its high molecular specificity and sensitivity are valuable in biomedical and clinical research.
- RTS applications include studying cell viability, deformation, and interactions.
Purpose of the Study:
- To review the principles and clinical applications of Raman tweezers spectroscopy (RTS) in transfusion medicine.
- To highlight RTS's utility in assessing blood product quality and storage effects.
Main Methods:
- Raman tweezers spectroscopy (RTS) combines optical tweezers with Raman spectroscopy.
- Analysis of Raman spectra from individual blood cells.
- In-situ study of live single cells in physiological environments.
Main Results:
- RTS detects changes in cellular blood components by analyzing Raman spectra.
- These spectral changes can monitor blood product quality during storage and transfusion.
- RTS provides insights into storage lesions and the impact of preservatives and fluids on blood cells.
Conclusions:
- RTS is a versatile tool for biomedical research, particularly in transfusion medicine.
- It enables the exploration of live single cell dynamics.
- RTS facilitates the monitoring of blood product integrity and safety.
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