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Updated: May 8, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
Published on: July 25, 2022
Electronically Controlled Dual-Wavelength Switchable SRS Fiber Amplifier in the NIR-II Region for Multispectral
Hwidon Lee1, Markus R Seeger2, Brett E Bouma3
1Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea; Engineering ResearchCenter forColor-Modulated Extra-Sensory Perception Technology, Pusan National University, Busan 46241, Republic of Korea; Harvard Medical School, Boston, MA 02114, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
A new fiber laser system enables efficient multispectral photoacoustic microscopy (PAM) by providing fast switching between near-infrared wavelengths. This breakthrough addresses a key limitation in advanced optical imaging technologies.
Area of Science:
- Biomedical Optics
- Laser Physics
- Materials Science
Background:
- Photoacoustic microscopy (PAM) offers high-resolution, non-invasive imaging with optical absorption contrast.
- Multispectral PAM requires light sources with high repetition and switching rates, microjoule pulse energy, and nanosecond durations.
- A gap exists in suitable light sources, especially for the near-infrared-II window.
Purpose of the Study:
- To develop and demonstrate a novel all-fiber-based light source for efficient multispectral photoacoustic microscopy.
- To address the need for a light source with specific parameters for advanced PAM applications.
Main Methods:
- Designed and constructed a custom all-fiber laser system.
- Utilized digitally addressed semiconductor seed lasers and stimulated Raman scattering amplification.
- Achieved arbitrary pulse sequences at 1168.4 nm and 1202.1 nm, with a 100 kHz switching rate between wavelengths.
Main Results:
- The developed light source delivers >3 μJ, 2 ns pulses at a 200 kHz repetition rate.
- Demonstrated arbitrary wavelength sequencing and 100 kHz switching between 1168.4 nm and 1202.1 nm.
- Successfully performed high-resolution multispectral photoacoustic microscopy on three polymer samples.
Conclusions:
- The custom all-fiber light source meets the stringent requirements for efficient multispectral PAM.
- This technology enables advanced spectroscopic analysis and high-resolution imaging in the near-infrared-II window.
- The demonstrated system is suitable for various applications requiring multispectral photoacoustic imaging.
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