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Published on: June 20, 2020
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High-throughput, combinatorial droplet generation by sequential spraying.
1Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, USA. njc222@cornell.edu.
Lab on a Chip
|January 2, 2025
Summary
Sequential spraying of fluorophore-encoded solutions creates combinatorial droplets for high-throughput biochemical assays. This novel method effectively analyzes antibiotic interactions by quantifying mixed droplet concentrations over time.
Area of Science:
- Biochemistry
- Biotechnology
- Analytical Chemistry
Background:
- Bulk and microfluidic emulsion methods advance biochemical assays.
- Spray-based techniques offer rapid droplet generation but are underutilized due to random, polydisperse droplets.
Purpose of the Study:
- To present a novel spray-based method for generating combinatorial droplets.
- To leverage droplet polydispersity for high-throughput biochemical analysis.
- To investigate pairwise antibiotic interactions using this new technique.
Main Methods:
- Sequential spraying of fluorophore-encoded solutions.
- Generation of mixed droplets to populate a combinatorial space.
- Quantification of droplet concentrations and imaging over time.
Main Results:
- Demonstrated a method for creating combinatorial droplets with quantifiable concentrations.
- Successfully applied the technique to investigate antibiotic interactions.
- Showcased the utility of spray-based methods for complex biological assays.
Conclusions:
- Sequential spraying offers a viable approach to high-throughput biochemical assays.
- This method enables the study of synergistic and antagonistic interactions.
- Leveraging droplet polydispersity expands the capabilities of spray-based assays.
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