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Urinary Bladder

Urinary Bladder

The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...

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Related Experiment Video

Updated: Jun 1, 2025

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Published on: August 9, 2012

An Acellular Platform to Drive Urinary Bladder Tissue Regeneration.

Mitali Kini¹, Matthew I Bury², Arun K Sharma³

¹Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Advanced Therapeutics

|January 17, 2025

Summary

This summary is machine-generated.

This study explored using cell-free total conditioned media (TCM) to regenerate bladder tissue, finding it promotes significant tissue regrowth and functional recovery. This offers a promising alternative to cell-seeded scaffolds for bladder augmentation.

Keywords:

cell-free therapy tissue regeneration urinary diversion wound healing

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Last Updated: Jun 1, 2025

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

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Published on: August 9, 2012

Surgical Model for Single-Staged Tissue-Engineered Urothelial Tubes in Minipigs

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Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

Published on: February 24, 2016

Area of Science:

Regenerative Medicine
Urology
Biomaterials

Background:

Impaired bladder compliance can cause kidney damage, often treated with intestinal augmentation, risking complications.
Current cell-seeded scaffolds for bladder regeneration face challenges in clinical cell acquisition.
Mesenchymal stem cell (MSC)/hematopoietic stem cell (HSPC) scaffolds show promise but require viable cell sourcing.

Purpose of the Study:

To evaluate the efficacy of cell-free total conditioned media (TCM) in promoting bladder tissue regeneration.
To compare TCM's regenerative capacity against media alone in an in-vivo rat model.
To assess functional bladder recovery using urodynamic measures post-augmentation.

Main Methods:

Partial cystectomy and augmentation with PRS scaffolds in immune-competent rats.
Instillation of TCM or control media twice weekly for 4 weeks.
Histological analysis of smooth muscle, urothelium, vascularization, and nerve regrowth; pre- and post-augmentation urodynamic studies.

Main Results:

TCM-instilled grafts exhibited enhanced smooth muscle content, urothelial width, and vascularization.
Greater peripheral nerve infiltration was observed in the TCM group.
Urodynamic studies showed improved bladder recovery and storage capacity in the TCM-treated group.

Conclusions:

Cell-free TCM effectively promotes bladder tissue regeneration and functional recovery.
TCM represents a viable, cell-free alternative to traditional cell-seeded scaffolds for bladder augmentation.
This approach could overcome clinical challenges associated with cell acquisition for regenerative therapies.