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Updated: May 30, 2025

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
Published on: August 8, 2019
Wide-area topography using reflection phase microscopy for surface inspection
Abstract:
In this study, we demonstrated an inspection system utilizing reflection phase microscopy to enhance both depth range and field of view (FOV). By implementing a dual-wavelength method, we achieved a maximum expected depth range of 7.7 µm. The amplified phase due to the dual-wavelength approach was effectively mitigated by utilizing the configuration of successive accumulation of interferograms. In addition, the error correction algorithms further enhanced image quality, yielding a precision of 1.30 nm. Furthermore, we employed sophisticated image stitching techniques to attain a FOV of approximately 11 × 11 mm2. This system enabled us to perform precise, wide-area inspections of hole structures fabricated on a silicon wafer, demonstrating its efficacy and potential for high-resolution, large-scale industrial applications.
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