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Updated: May 29, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
The Mass Spectrometry Revolution in Clinical Microbiology Part 2: Emerging Applications
1Division of Geographic Medicine and Infectious Diseases, Tufts University School of Medicine, Boston, MA, USA; Department of Pathology and Laboratory Medicine, Tufts University School of Medicine, Tufts Medical Center, 800 Washington Street #115, Boston, MA 02111, USA.
Abstract:
In part 2 of this series, we highlight some of the exciting and emerging applications of mass spectrometry (MS) in pathogen detection, identification, and characterization. First, we review applications that have been recently introduced into the clinical microbiology laboratory such as direct matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) from positive blood cultures as well as its incorporation into laboratory automation. We then explore how current MALDI-TOF platforms are being developed to determine antimicrobial resistance and strain typing. Finally, we showcase some emerging approaches such as MS imaging and non-MALDI-based MS techniques such as ambient ionization and breath-based pathogen detection.
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