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Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
Published on: May 9, 2018
Uncovering Novel Drugs that Restore Vision Using Orthogonal Pooling in Zebrafish
Justine O'Brien1,2, Patrizia Colucci1,2, Yolanda Alvarez1,2
1UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland.
Abstract:
Photoreceptor and retinal pigment epithelium (RPE) dysfunction in inherited retinal degenerations (IRDs) and age-related macular degeneration (AMD) necessitate innovative therapies to preserve vision. Vision impairment incurs a substantial global economic burden, with the World Health Organization reporting an annual global productivity loss of approximately $411 billion. Current treatments are limited, underscoring the urgency for novel solutions. Leveraging new screening techniques, novel drugs restoring vision can be uncovered. Here, a workflow is described utilising orthogonal pooling to screen randomised library compounds for drug hits restoring vision and assessing the optokinetic response (OKR) in the atp6v0e1-/- zebrafish model of inherited blindness.
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