Quantification of Pectolinarin in Rat Plasma Using UPLC-MS/MS and Its Pharmacokinetic Analysis

Area of Science:

• Pharmacology and Pharmacokinetics
• Analytical Chemistry
• Natural Product Chemistry

Background:

• Pectolinarin, a flavonoid, exhibits wound-healing, anti-inflammatory, and antibacterial properties.
• Understanding the pharmacokinetic profile of pectolinarin is crucial for its therapeutic development.

Purpose of the Study:

• To develop and validate a UPLC-MS/MS method for quantifying pectolinarin in rat plasma.
• To investigate the pharmacokinetic characteristics of pectolinarin following oral and intravenous administration in rats.

Main Methods:

• Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS) was utilized for quantification.
• Acetonitrile precipitation was used for plasma sample preparation.
• Electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode with positive ionization was employed for detection.

Main Results:

• The validated UPLC-MS/MS method demonstrated excellent linearity (r > 0.995) over a concentration range of 1.2–2300 ng/mL.
• High accuracy (97.3%–108.3%) and precision (RSD < 9.2%) were achieved, with minimal matrix effects (97.8%–105.3%).
• The absolute oral bioavailability of pectolinarin in rats was determined to be 0.28%.

Conclusions:

• A robust and sensitive UPLC-MS/MS method for pectolinarin analysis in rat plasma was successfully developed and validated.
• The study provides essential pharmacokinetic data for pectolinarin, highlighting its limited oral absorption in rats.