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Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
Published on: May 12, 2023
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Metal-Phenolic Network Hydrogel Vaccine Platform for Enhanced Humoral Immunity against Lethal Rabies Virus
Jiamin Deng1, Zongmei Wang2, Liqin Wu2
1The National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, College of Chemistry, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
ACS Nano
|March 3, 2025
Summary
A novel single-dose hydrogel vaccine (CGMR) effectively prevents rabies by boosting neutralizing antibodies. This manganese-based vaccine offers a promising, cost-effective solution for global infectious disease prevention.
Area of Science:
- Biomaterials Science
- Immunology
- Vaccinology
Background:
- Rabies virus (RABV) poses a significant global health threat, with eradication efforts hampered by the high cost and multiple doses required for current vaccines.
- Developing single-dose vaccines is crucial for improving accessibility, especially in developing nations.
Purpose of the Study:
- To develop a novel single-dose vaccine for rabies prevention using a metal-phenolic network (MPN)-based hydrogel.
- To evaluate the immunogenicity and efficacy of the MPN-based hydrogel vaccine (CGMR) against RABV infection.
Main Methods:
- Fabrication of an MPN-based hydrogel vaccine (CGMR) using phenol-modified chitosan and manganese ions.
- Assessment of CGMR's ability to act as a hydrogel antigen depot and prolong antigen residence time.
- Evaluation of the vaccine's impact on the cGAS-STING pathway, dendritic cell maturation, and germinal center activation.
- Measurement of virus-neutralizing antibody levels and protection against virulent RABV challenge.
Main Results:
- The CGMR vaccine induced significantly enhanced virus-neutralizing antibody responses (4.3-fold and 1.8-fold increases) compared to inactivated RABV and alum adjuvant, respectively.
- The hydrogel structure prolonged antigen presence and activated the cGAS-STING pathway, promoting immune cell recruitment and activation.
- Single immunization with CGMR provided robust and sustained humoral immunity, protecting against lethal RABV challenge.
Conclusions:
- The injectable manganese-based hydrogel vaccine (CGMR) represents a promising single-dose strategy for effective rabies prevention.
- This platform technology offers a universal and potentially cost-effective approach for developing vaccines against rabies and other infectious diseases.
