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A new dielectric stack for digital microfluidic chips ensures 60-day stability in incubators. This durable solution enables precise long-term cell culture assays, including drug testing and cytokine analysis.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • Digital microfluidic (DMF) devices are valuable for cell-based assays.
  • Incubator humidity poses challenges for DMF device longevity.
  • Robust dielectric layers are crucial for reliable DMF operation in biological applications.

Purpose of the Study:

  • To develop and characterize a durable multilayer dielectric stack for DMF chips.
  • To assess the stack's performance under humid cell culture conditions.
  • To demonstrate the utility of the DMF platform with the new dielectric stack for long-term biological assays.

Main Methods:

  • Fabrication of a multilayer dielectric stack using polyvinylidene difluoride (PVDF) and SU-8.
  • Electrical characterization including breakdown voltage and surface current measurements.
  • Long-term stability testing in a humid cell culture incubator (60 days).
  • Application in macrophage cell culture assays, including phenotype modulation and drug testing.

Main Results:

  • The dielectric stack exhibited high breakdown voltages (up to 1600 V) and low surface currents (<30 nA at 100 V).
  • The stack maintained integrity and functionality for over 60 days in humid incubator conditions.
  • The DMF platform enabled single-cell cytokine quantification and assessment of drug TCB-2 effects on macrophages.

Conclusions:

  • The developed multilayer dielectric stack provides a robust and durable solution for DMF devices.
  • This advancement facilitates reliable, long-term biological assays in humid environments.
  • The DMF platform with the new dielectric stack is suitable for studying cellular responses and drug efficacy.