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High-dimensional signalling analysis of organoids.
Aurélie Dobric1, Christopher J Tape1
1Cell Communication Lab, Department of Oncology, University College London Cancer Institute, 72 Huntley Street, London, WC1E 6DD, UK.
Current Opinion in Cell Biology
|March 12, 2025
Summary
Organoids enable large-scale studies of cell signaling. Advanced single-cell and multimodal analyses reveal complex signaling mechanisms driving cell fate dynamics and coordination across biological layers.
Area of Science:
- Cellular biology
- Biotechnology
- Systems biology
Background:
- Cellular phenotypes are governed by intricate signaling pathways.
- Understanding these pathways is crucial for deciphering cell behavior and disease.
- Organoids offer a scalable, biomimetic platform for studying cellular processes.
Purpose of the Study:
- To review recent advancements in high-dimensional analysis of cell signaling within organoid models.
- To highlight the utility of organoids in functional perturbation studies.
- To discuss how emerging technologies enhance mechanistic understanding of cell signaling.
Main Methods:
- High-dimensional analysis of organoid signaling.
- Application of single-cell technologies for cell-type specific analysis.
- Utilizing emerging multimodal techniques to capture integrated signaling data.
Main Results:
- Single-cell analyses provide detailed insights into cell signaling and cell-fate dynamics.
- Multimodal techniques reveal cross-talk and coordination between different signaling layers.
- Organoids facilitate large-scale perturbation studies for dissecting signaling networks.
Conclusions:
- Organoids are powerful models for high-dimensional cell signaling analysis.
- Advanced analytical techniques are deepening our understanding of signaling mechanisms.
- Future research will further elucidate the coordination of signaling layers for mechanistic insights.
