Related Experiment Video
Updated: May 22, 2025
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
A Comparative Analysis of Transfected and Integrated Auxin Reporter Systems Reveals Sensitivity Advantages in
Joseph S Taylor1, Eric A Villaseñor1, James Rashkovsky1
1School of Plant and Environmental Sciences, Virginia Tech, Blacksburg, Virginia, United States.
None:
Reporter-gene activation studies using transient transformation of protoplasts are a powerful tool for the investigation of transcriptional regulation in plants. Here, we perform a comparative analysis of reporter-gene activation sensitivity using an integrated versus a co-transfected reporter-gene construct in Arabidopsis seedling mesophyll protoplasts. The DR5 synthetic auxin-responsive promoter was used to assay the response to auxin treatment and over-expression of activator Auxin Response Factors. We show that sensitivity, as measured by the fold-change in fluorescent-protein reporter-gene expression, is significantly increased by using a co-transfected reporter-gene construct.
More Related Videos
08:21Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation BiFC System
Published on: September 16, 2011
09:41Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Published on: May 14, 2020
