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Updated: May 21, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

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Published on: January 14, 2021

Vascular network-inspired diffusible scaffolds for engineering functional midbrain organoids.

Hongwei Cai¹, Chunhui Tian¹, Lei Chen¹

¹Department of Intelligent Systems Engineering, Indiana University Bloomington, Bloomington, IN 47405, USA.

|March 18, 2025

Summary

This summary is machine-generated.

Vascular network-inspired diffusible scaffolds improve organoid function by mimicking physiological diffusion, reducing necrosis and hypoxia for better drug response studies.

Keywords:

diffusion drug response electrophysiology organoids perfusion scaffolds vascular networks

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Last Updated: May 21, 2025

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Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Area of Science:

Biotechnology
Developmental Biology
Neuroscience

Background:

Organoids, derived from stem cells, offer potential in various fields but face limitations in nutrient and oxygen diffusion.
Neural organoids, in particular, suffer from functional and phenotypic constraints due to poor diffusion.

Purpose of the Study:

To develop and evaluate vascular network-inspired diffusible (VID) scaffolds for enhanced organoid engineering.
To improve the physiological relevance and drug-response phenotyping of organoids, particularly neural organoids.

Main Methods:

3D-printing of meshed tubular channel networks (VID scaffolds) to mimic physiological diffusion.
Engineering human midbrain organoids using VID scaffolds in standard well plates.
Comparing engineered organoids with conventional organoids regarding necrosis, hypoxia, and physiological features.

Main Results:

VID scaffolds successfully engineered human midbrain organoids with minimal necrosis and hypoxia.
Engineered organoids exhibited enhanced midbrain-specific identity, oxygen metabolism, neuronal maturation, and network activity.
Organoids engineered with VID scaffolds showed improved recapitulation of pharmacological responses to fentanyl compared to conventional organoids.

Conclusions:

VID scaffolds overcome diffusion limitations in organoid culture, enabling the generation of more functional and physiologically relevant organoids.
This platform enhances organoid drug response phenotyping, offering a promising tool for therapeutic innovation and developmental biology research.