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Related Concept Videos

Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

12.1K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
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Mechanically-gated Ion Channels01:12

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

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Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
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Related Experiment Video

Updated: May 21, 2025

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
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Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology

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Modulating ion channels with nanobodies.

Sher Ali1, Ashley Suris2, Yun Huang2,3

  • 1Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA.

Synthetic and Systems Biotechnology
|March 19, 2025
PubMed
Summary
This summary is machine-generated.

Nanobodies, derived from camelids, are emerging as powerful tools for studying ion channels. These small, specific antibody fragments offer new ways to understand and potentially treat diseases related to ion channel function.

Keywords:
Antibody engineeringImmunotherapyIon channelsNanobodySynthetic biologyTherapeutics

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Ion channels are crucial for cellular functions and disease.
  • Understanding ion channel structure-function is key for therapeutics.

Purpose of the Study:

  • To review the application of nanobodies in ion channel research.
  • To highlight nanobodies' potential in overcoming limitations of current methods and drugs.

Main Methods:

  • Review of recent literature on nanobody applications in ion channel biology.
  • Analysis of nanobody advantages over conventional antibodies and biologics.

Main Results:

  • Nanobodies offer high specificity and can target difficult epitopes.
  • Their small size and low immunogenicity are advantageous.
  • Nanobodies provide new avenues for exploring ion channel structure-function relationships.

Conclusions:

  • Nanobodies represent a promising tool for ion channel research and therapeutic development.
  • They offer a potential alternative to conventional drugs, addressing issues like off-target effects and toxicity.