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Post-Treatment Apical Periodontitis: Endodontic Infection Contributes to Circulating Microbial DNA.

Yanglin Zeng1, Liu Wang1,2, Mudan Wang3

  • 1State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

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Summary
This summary is machine-generated.

Post-treatment apical periodontitis translocates unique microbial DNA into the blood. The root apex and periapical lesions significantly contribute to this bloodstream microbial presence in patients.

Keywords:
circulating microbial DNAendodontic infectionpost‐treatment apical periodontitis

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Area of Science:

  • Microbiology
  • Endodontics
  • Genomics

Background:

  • Apical periodontitis is an endodontic disease affecting the root apex.
  • Understanding microbial translocation in post-treatment apical periodontitis is crucial for patient health.

Purpose of the Study:

  • To investigate the impact of post-treatment apical periodontitis on microbial DNA in blood circulation.
  • To identify specific microbial signatures and their origins in patients' blood.

Main Methods:

  • Next-generation sequencing of microbial DNA from root apex, periapical lesions, and peripheral blood.
  • Analysis of microbial composition, diversity, and SourceTracker for microbial tracing.
  • Comparison between 11 patients with post-treatment apical periodontitis and 9 healthy controls.

Main Results:

  • Proteobacteria dominated peripheral blood and periapical lesions; Actinobacteria prevailed in the root apex.
  • Patients showed higher blood microbial diversity but reduced root apex α-diversity.
  • Specific bacteria like Bacteroides, Aliihoeflea, and Shigella were more abundant in patient blood.
  • Root apex and periapical lesions contributed 26% and 76% to peripheral blood bacteria, respectively.

Conclusions:

  • Post-treatment apical periodontitis is associated with unique microbial translocation into the bloodstream.
  • The periapical lesion is a major source of bacteria found in the peripheral blood of these patients.
  • Findings highlight the systemic impact of endodontic infections.