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Updated: Jul 11, 2026
Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
Published on: June 9, 2016
Protocol for biomimetic tumoroid models by plastic compression using centrifugation
Sam Devereaux1, Ashley Lam1, Anuja Upadhyay1
1UCL Centre for 3D Models of Health and Disease, UCL Division of Surgery and Interventional Science, Faculty of Medical Sciences, Charles Bell House, University College London, 43-45 Foley Street, London W1W 7TY, UK.
Abstract:
Here, we present a protocol for engineering biomimetic tumoroid models by plastic compression using centrifugation. We describe steps for generating multi-compartment tumor-stroma models by mixing cells into a collagen hydrogel crosslinked at 37°C and centrifuging the hydrogel. We then detail procedures for generating compartmentalized models and encapsulating the final layered hydrogel containing a 96-well tumor mass in a 24-well stroma. This protocol increases collagen density and improves mechanical properties of collagen hydrogels.
