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Updated: May 9, 2025
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
Published on: March 10, 2023
A Non-destructive, Image Analysis Method for Evaluating Pigmentation in Induced Pluripotent Stem Cell-Derived Retinal
Gopika S Kumar1, Gowri P Chidambaranathan1, Anwar A Palakkan2
1Immunology & Stem Cell Biology, Aravind Medical Research Foundation, Madurai, Tamil Nadu, India.
Abstract:
We have developed an image J macro to analyze pigmentation level in iPSC-derived retinal pigment epithelial cells from bright-field images. Human iPSC-derived retinal pigment epithelial cells were treated with hydroquinone to induce hyperpigmentation, and untreated cells were used as controls for the study. The Image analysis results were validated by estimating melanin spectrophotometrically, after lysing the cells. The developed image analysis method will be useful for screening drugs/compounds that induce hyper/hypo pigmentation in cultured iPSC-derived RPE cells.
