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Three-Dimensional-Printed Flexible Nanosilver Electrode Array for Parallel and Robust Intracellular
Keda Shi1,2,3, Liang Hu4, Duote Cai1
1General Surgery Department, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children's Health, Hangzhou 310052, China.
ACS Nano
|May 14, 2025
Summary
Researchers developed a 3D-printed flexible nanosilver electrode array (FlexNEA) for high-fidelity cardiac electrophysiology recordings. This advanced device improves intracellular access and drug screening for cardiovascular research.
Area of Science:
- Cardiac electrophysiology
- Biomedical engineering
- Nanotechnology
Background:
- Intracellular action potential (iAP) recordings are crucial for understanding and treating cardiovascular diseases.
- Conventional electrodes face limitations in signal quality and ease of use for iAP recordings.
Purpose of the Study:
- To develop a novel 3D-printed flexible nanosilver electrode array (FlexNEA) for enhanced cardiac electrophysiology.
- To evaluate the FlexNEA's performance in intracellular access, signal quality, and drug screening applications.
Main Methods:
- Utilized a multimaterial electric-field-driven (EFD) micro-jet 3D printing strategy for rapid electrode fabrication.
- Employed electroporation for efficient and biosafe intracellular access.
- Assessed cell-electrode coupling and signal fidelity compared to conventional electrodes.
Main Results:
- Achieved over 99% success rate in intracellular access via electroporation.
- Demonstrated superior signal quality due to enhanced cardiomyocyte-electrode coupling with the flexible NEA.
- Enabled stable, high-fidelity intracellular recordings and accurate drug-induced iAP alteration detection for drug screening.
Conclusions:
- The 3D-printed FlexNEA offers a significant advancement in high-fidelity intracellular recording for cardiac electrophysiology.
- The integrated platform provides a precise, quantitative method for assessing ion-channel drug effects.
- Presents a low-cost, biocompatible solution for preclinical cardiology and pharmacology research.
