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Updated: May 23, 2025
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
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CRISPR-Based Regulation for High-Throughput Screening.
Lingling Jiao1, Qi Zhou1, Dongchang Sun1
1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.
ACS Synthetic Biology
|May 22, 2025
Summary
CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) offer reversible gene regulation, enabling powerful genome-wide screening for biomedical and industrial applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR technology enables precise genome editing but often results in irreversible changes.
- CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) were developed to provide reversible gene regulation.
- These methods utilize inactivated Cas proteins and guide RNA for gene silencing or activation.
Purpose of the Study:
- To review CRISPR regulation mechanisms for gene silencing and activation.
- To provide an overview of genome-wide CRISPR-based screening workflows.
- To highlight the suitability of CRISPRi/a for high-throughput screening (HTS) in various applications.
Main Methods:
- Exploration of CRISPRi/a mechanisms for gene regulation.
- Description of genome-wide CRISPR screening workflows.
- Review of applications in high-throughput screening (HTS).
Main Results:
- CRISPRi/a technologies allow for precise, reversible gene modulation.
- Genome-wide CRISPR-based screening facilitates identification of genetic determinants.
- CRISPRi/a HTS is highly suitable for biomedical and industrial applications.
Conclusions:
- CRISPRi/a significantly expands the utility of CRISPR technology for research.
- CRISPR-mediated HTS offers a powerful approach for discovery across diverse fields.
- Future directions include addressing limitations and broadening applications of CRISPRi/a HTS.
