Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

1.7K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
1.7K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

1.1K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.1K
Precipitate Formation and Particle Size Control01:16

Precipitate Formation and Particle Size Control

751
In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
751
GTPases and their Regulation02:14

GTPases and their Regulation

8.3K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
8.3K
Polymer Classification: Crystallinity01:21

Polymer Classification: Crystallinity

2.8K
Unlike ionic or small covalent molecules, polymers do not form crystalline solids due to the diffusion limitations of their long-chain structures. However, polymers contain microscopic crystalline domains separated by amorphous domains.
Crystalline domains are the regions where polymer chains are aligned in an orderly manner and held together in proximity by intermolecular forces. For example, chains in the crystalline domains of polyethylene and nylon are bound together by van der Waals...
2.8K
Colloidal precipitates01:09

Colloidal precipitates

524
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
524

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Damselflies overcome color saturation barriers of photonic glasses via pigment loading and refractive index modulation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Control Strategies in Guanine Biocrystallization.

Angewandte Chemie (International ed. in English)·2026
Same author

Committor-regularized learning of differentiable collective variables from non-differentiable structural descriptors.

The Journal of chemical physics·2026
Same author

The quasi-liquid layer thickness controls clathrate hydrates' growth rate.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Unsupervised Classification of Local Clathrate Hydrate Structures.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Publisher's Note: "'Ensemblization' of density functional theory" [J. Chem. Phys. 164, 040901 (2026)].

The Journal of chemical physics·2026
Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026
Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026
Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026
Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2025

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
08:58

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Published on: July 5, 2018

12.6K

Guanine Crystallization by Particle Attachment.

Shashanka S Indri1, Florian M Dietrich2, Avital Wagner1

  • 1Department of Chemistry, Ben-Gurion University of the Negev, Be'er Sheba 8410501, Israel.

Journal of the American Chemical Society
|May 23, 2025
PubMed
Summary
This summary is machine-generated.

Researchers uncovered guanine crystallization by combining molecular dynamics simulations and experiments. This reveals a multi-step nonclassical nucleation process, crucial for understanding biological crystal formation.

More Related Videos

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.2K
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

10.0K

Related Experiment Videos

Last Updated: Jun 12, 2025

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
08:58

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Published on: July 5, 2018

12.6K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.2K
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

10.0K

Area of Science:

  • Materials Science
  • Biomineralization
  • Chemistry

Background:

  • Crystallization nucleation is vital across multiple scientific disciplines.
  • Nonclassical crystallization via particle attachment is common in inorganic materials.
  • Molecular-level insights into small molecule nucleation are limited by simulation and observation challenges.

Purpose of the Study:

  • To elucidate the nonclassical crystallization mechanism of guanine at the molecular level.
  • To bridge the gap in understanding nucleation events that are too large to simulate and too small to observe.
  • To investigate the multi-step nucleation process of guanine.

Main Methods:

  • Unbiased molecular dynamics simulations.
  • In situ experimental observations.
  • Electron microscopy for nanoscopic fiber visualization.

Main Results:

  • Identified a multi-step nucleation pathway for guanine.
  • Observed the formation of stacked guanine clusters that progressively order.
  • Documented the assembly into nanoscopic fibers, ordered bundles, and finally 3D crystals.

Conclusions:

  • The study elucidates the nonclassical crystallization mechanism of guanine.
  • Provides a foundation for understanding biological control over guanine crystal formation.
  • Highlights the role of guanine crystals in biological optical and nitrogen-storage functions.