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Gold disk microelectrode-coupled label-free electrochemical aptasensor for dopamine assay.
Sunying Yang1, Jiaqi Cai2, Liu Su2
1School of Chemistry, Engineering Research Center of Energy Storage Materials and Devices, Ministry of Education of China, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China. hetongqi@xjtu.edu.cn.
The Analyst
|June 25, 2025
Summary
We developed a novel label-free electrochemical aptasensor using a gold disk microelectrode for sensitive and selective dopamine detection in brain slices. This biosensor successfully monitored dopamine dynamics in real-time within living brain tissue.
Area of Science:
- Electrochemistry
- Neuroscience
- Biosensor technology
Background:
- Dopamine (DA) plays a crucial role in neurological functions and diseases.
- Accurate detection of DA in brain tissue is essential for understanding neurochemical dynamics.
- Existing methods for DA detection often face limitations in sensitivity, selectivity, or spatial resolution.
Purpose of the Study:
- To develop a label-free electrochemical aptasensor for sensitive and selective dopamine detection.
- To fabricate a gold disk microelectrode (Au DME) for enhanced biosensor performance.
- To demonstrate the application of the aptasensor in monitoring real-time DA changes in brain slices.
Main Methods:
- Fabrication of a gold disk microelectrode (Au DME) with controlled geometry.
- Development of a label-free electrochemical aptasensor by immobilizing an anti-DA aptamer on Au DME.
- Detection of dopamine based on its electrochemical oxidation signal.
- Application of the aptasensor in mouse striatal slices to record stimulated DA release.
Main Results:
- The aptasensor exhibited high sensitivity for dopamine detection, with a wide linear range (0.5–27 μM) and a low detection limit (0.11 μM).
- The biosensor demonstrated excellent selectivity, accurately detecting dopamine in the presence of interfering neurochemicals.
- The developed aptasensor successfully monitored dynamic changes in dopamine concentration in living brain tissue.
Conclusions:
- The label-free electrochemical aptasensor based on Au DME offers a promising tool for high-resolution, sensitive, and selective determination of neurochemicals.
- This approach provides a valuable strategy for studying neurochemical dynamics in living systems.
- The fabricated microelectrode-based aptasensor holds potential for future neuroscience research and clinical applications.
