Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

534
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
534
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

604
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
604
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hidden in visible light: spectral-temporal unmixing of lung tissue autofluorescence in a fibre-based system.

Biomedical optics express·2026
Same author

Assessing liquid light guides in diffuse correlation spectroscopy systems.

Biomedical optics express·2025
Same author

Fiber-Based Ultra-High-Speed Diffuse Speckle Contrast Analysis System for Deep Blood Flow Sensing Using a Large SPAD Camera.

Biosensors·2025
Same author

Cerebral blood flow monitoring using a deep learning implementation of the two-layer diffuse correlation spectroscopy analytical model with a 512 × 512 SPAD array.

Neurophotonics·2025
Same author

Real-time detection of singlet-oxygen signatures using a single-photon avalanche diode detector.

Biomedical optics express·2025
Same author

ATLAS: a large array, on-chip compute SPAD camera for multispeckle diffuse correlation spectroscopy.

Biomedical optics express·2024
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Sep 15, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.2K

Time-resolved Raman spectroscopy using a CMOS SPAD array to remove fluorescent and fibre Raman backgrounds.

Caitlin S Tye1, András Kufcsák1, Calum A Ross1

  • 1Scottish Universities Physics Alliance (SUPA), Institute of Photonics and Quantum Sciences, Heriot-Watt University, Edinburgh, UK.

Biomedical Optics Express
|July 18, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new CMOS SPAD sensor for enhanced Raman spectroscopy, significantly improving signal detection and suppressing background noise for faster, more sensitive measurements.

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.1K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K

Related Experiment Videos

Last Updated: Sep 15, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.2K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.1K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.3K

Area of Science:

  • Spectroscopy
  • Optical Engineering
  • Materials Science

Background:

  • Raman spectroscopy faces challenges with weak signals, fluorescence interference, and fiber-induced backgrounds.
  • Existing methods struggle to efficiently detect faint Raman signals while mitigating noise sources.

Purpose of the Study:

  • To develop a compact optical system for enhanced Raman signal detection.
  • To suppress background fluorescence and fiber-induced signals using time-gating techniques.
  • To enable miniaturized single-fiber Raman probes for remote sensing applications.

Main Methods:

  • Utilized a 512-pixel complementary-metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) line sensor array.
  • Integrated on-chip timing electronics for precise signal detection and time-gating.
  • Demonstrated functionality in free-space and through a 1m 50µm core multimode fiber.

Main Results:

  • Achieved improved signal enhancement with significantly shorter measurement times (30 s) in free space.
  • Successfully suppressed fiber background noise for remote detection of samples like paracetamol.
  • Validated the system's capability for miniaturized single-fiber Raman probe development.

Conclusions:

  • The developed CMOS SPAD sensor array effectively enhances Raman signal detection and background suppression.
  • Time-gating is a viable strategy for mitigating fluorescence and fiber-induced interference in Raman spectroscopy.
  • This technology paves the way for compact, high-performance fiber-based Raman probes for diverse applications.