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Updated: Sep 14, 2025
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
Published on: June 12, 2013
EFFECTS OF HYPERBARIC, HYPEROXIA, PRESSURE AND HYPOXIA ON CD38 AND CD157 EXPRESSION IN ISOLATED PERIPHERAL BLOOD
Z Al-Abady1, N Jabbar1, S Hamzah2
11Department of Chemistry, College of Science, University of Al-Qadisiyah, Iraq.
Background:
Oxygen therapy, utilizing both normal and elevated pressures, is a standard treatment for a range of medical conditions. Given that administered oxygen impacts the entire body, including blood constituents, this research examines how different oxygen and pressure environments influence gene expression in human peripheral blood monocytes.
Methods:
The treatment of isolated PBM with hyperbaric oxygen (HBO), hyperoxia, elevated pressure and hypoxia were performed. In addition, the expression of different Monocytes surface epitopes (CD38 and CD157 expression) were examined by qPCR technique. Normoxic culture media served as a positive control.
Results:
The impact of 90-minute exposures to hyperbaric oxygen, hyperoxia, and hypoxia on CD38 and CD157 expression in monocytes was investigated. Compared to normoxic controls, both hyperbaric oxygenation and hyperoxia significantly decreased (p < 0.05) CD38 and CD157 expression. Notably, CD157 expression exhibited a greater reduction under hyperbaric oxygenation than CD38. Conversely, hypoxia induced a significant increase in CD38 expression, while simultaneously reducing CD157 expression relative to normoxia.
Conclusions:
Changes in oxygen levels and pressure, specifically through hyperbaric oxygen, hyperoxia, and hypoxia treatments, demonstrably alter monocyte behavior and the expression of surface epitopes. These alterations could play a significant role in conditions where monocytes are implicated. The data aligns with existing research highlighting the influence of oxygen tension on cellular proliferation and protein production. Consequently, these findings underscore the potential importance of controlled oxygen administration, whether normobaric or hyperbaric, in clinical settings.
