A surface-imprinting lanthanide fluorescent hybrid probe on SiO₂ microspheres for the detection of enrofloxacin

A surface-imprinted polymer SiO₂@Eu(DBM)₃phen/SMIP has been synthesized using enrofloxacin (ENFX) as the template molecule, methacrylic acid as the functional monomer, and the complex Eu(DBM)₃phen as a fluorescent moiety. The structure and the fluorescence sensing properties of SiO₂@Eu(DBM)₃phen/SMIP have been investigated in detail. The resulting material exhibited high selectivity, a low detection limit of 3.07 µM, and a linear working range from 20 to 100 µM for ENFX. The obtained fluorescence probe demonstrates good selectivity and sensitivity toward ENFX and has the potential for significant application in ENFX detection by simplifying procedures and reducing interference from impurities. In addition, the detection mechanism of the fluorescent probe was also discussed. The results indicate that it is feasible to use SiO₂@Eu(DBM)₃phen/SMIP for the detection of ENFX as well as its effective adsorption. This study explores the self-assembly of rare-earth complexes on silica via a surface imprinting technique, which provides a new solution for the subsequent development of the surface blotting technique.