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Micro-hardness of root canal dentin using various irrigating solutions: An in vitro study
Richa Pathak1, Meetu Mathur2, Diganta Kumar Borah3
1Department of Conservative Dentistry and Endodontics, Buddha Institute of Dental Sciences and Hospital, Patna, Bihar, India.
Bioinformation
|August 18, 2025
Summary
This study found that 2% Chlorhexidine (CHX) is a safe root canal irrigant. Unlike 17% EDTA and 3% Sodium Hypochlorite, CHX did not significantly reduce dentin microhardness.
Area of Science:
- Endodontics
- Dental Materials Science
Background:
- Root canal irrigation is crucial for effective disinfection.
- The effect of irrigants on dentin microhardness is a significant concern for long-term tooth integrity.
Purpose of the Study:
- To evaluate the impact of common endodontic irrigants on dentin microhardness.
- To determine the safety of 2% Chlorhexidine Gluconate (CHX) as a root canal irrigant.
Main Methods:
- Eighty extracted mandibular premolars were used.
- Dentin microhardness was measured using a Vickers tester after immersion in Normal Saline, 3% Sodium Hypochlorite, 17% EDTA, or 2% CHX for 15 minutes at 37°C.
Main Results:
- All irrigants, except Normal Saline, reduced dentin microhardness.
- 17% EDTA caused the most significant decrease in microhardness.
- 2% CHX did not significantly affect dentin microhardness compared to Normal Saline.
Conclusions:
- 2% Chlorhexidine Gluconate is a suitable root canal irrigant.
- 17% EDTA and 3% Sodium Hypochlorite may negatively impact dentin integrity.
- Dentin microhardness is a key factor in selecting endodontic irrigants.
