Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Improving the culture surrounding tracheal aspirates in mechanically ventilated children.

Journal of clinical microbiology·2026

Same author

"Always over their shoulder": Educator and resident perceptions of supervision and autonomy in pediatric surgery rotations.

American journal of surgery·2026

Same author

Survival trends in the management of carcinoid heart disease among patients with neuroendocrine neoplasms.

Cardio-oncology (London, England)·2026

Same author

Surgical Education Feedback Culture and Future Considerations.

Journal of the American College of Surgeons·2026

Same author

Association between sex hormones and innate cytotoxic capacity of circulating and rectal mucosal immune cells.

Clinical immunology communications·2026

Same author

Altering PTPRD via genetics or pharmacology modulates 3xTg-AD mouse neurofibrillary pathology.

Frontiers in neuroscience·2026

Same journal

An Automated Atom Probe Tomography Cluster Detection Approach Using Transfer Learning.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Correlative Light and Electron Microscopy Visualization of Helicobacter pylori in Human Saliva.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Integrating Morpho-Anatomy and Histochemistry to Characterize Native Brazilian Eugenia Species.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

Same journal

Postnatal Developmental Expression and Localization of Water Channel Proteins in the Rat Ovary.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

Laboratory-Based Soft X-Ray Tomography: A Novel Laser Design, Source Monitoring, and Data-Processing Workflow.

Aurélie Dehlinger^1,2, Valentina Alberini^1,2, Vladimir Usatikov^1,2

¹Technische Universität Berlin, Institut für Physik und Astronomie, Hardenbergstr. 36 Berlin 10623, Germany.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|August 19, 2025

Summary

This summary is machine-generated.

Upgrades to the laboratory soft X-ray microscope (L-TXM) plasma chamber and data analysis improve performance. This advancement enhances three-dimensional imaging and segmentation for X-ray microscopy applications.

Keywords:

3D reconstruction image segmentation laboratory soft X-ray tomography laser-plasma source water-window microscopy

More Related Videos

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Related Experiment Videos

Last Updated: Jun 28, 2026

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Dynamic Pore-scale Reservoir-condition Imaging of Reaction in Carbonates Using Synchrotron Fast Tomography

Published on: February 21, 2017

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Area of Science:

Physics
Microscopy
Materials Science

Background:

Laboratory-based X-ray microscopy requires concurrent development of X-ray sources.
The water-window range is crucial for biological and materials imaging.
Existing systems necessitate improvements in robustness and user-friendliness.

Purpose of the Study:

To present major upgrades to the laboratory soft X-ray microscope (L-TXM) plasma chamber and data analysis protocol.
To demonstrate improved performance of the laser-plasma source.
To showcase the system's capability for 3D imaging and segmentation.

Main Methods:

Upgraded the L-TXM plasma chamber.
Developed a new data analysis protocol.
Characterized the laser-plasma source performance.
Acquired a proof-of-principle tomogram of a diatom.

Main Results:

Demonstrated improved performance of the laser-plasma source.
Showcased a robust data treatment protocol.
Successfully performed 3D imaging and segmentation of a diatom.
Validated the enhanced capabilities of the L-TXM system.

Conclusions:

The upgrades significantly enhance the L-TXM's performance and robustness.
The developed data analysis protocol is effective for 3D imaging and segmentation.
These advancements make L-TXM a more user-friendly tool for soft X-ray microscopy.